Membrane Anchored TNFα Cell (Adherent)
CBP74152
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| I. Background | |
| Transmembrane TNF-α, a precursor of the soluble form of TNF-α, is expressed on activated macrophages and lymphocytes as well as other cell types. After processing by TNF-α-converting enzyme (TACE), the soluble form of TNF-α is cleaved from transmembrane TNF-α and mediates its biological activities through binding to Types 1 and 2 TNF receptors (TNF-R1 and -R2) of remote tissues.Transmembrane TNF-α acts as a ligand by binding to TNF-α receptors as well as functioning as a receptor that transmits outside-to-inside (reverse) signals back into the transmembrane TNF-α-bearing cells (TNF-α-producing cells) . It is therefore considered that transmembrane TNF-α plays a critical role in local inflammation. | |
| II. Introduction | |
| Expressed gene: | Membrane Anchored TNFα |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+5ug/ml puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| III. Representative Data | |
Figure 1. Membrane Anchored TNFα Cell (Adherent) flow cytometry data.
Figure 2. Dose response of TNFα Abs in ADCC Bioassay Effector Cell (High Affinity/NFAT-Jurkat cells) With Membrane Anchored TNFα Cells (Adherent, Clone 12).
