NKG2D Effector Reporter Cell
CBP74222
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| I. Background | |
| Natural killer (NK) cells are innate lymphocytes with cytotoxic functions and recognise target cells with the NK group 2D (NKG2D) receptor. Tumor cells are marked for NK‐cell‐mediated destruction upon expression of MICA and MICB (MICA/B), which are NKG2D ligands upregulated by many human cancers in response to cellular stress pathways associated with malignant transformation such as DNA damage and accumulation of misfolded proteins. | |
| II. Introduction | |
| Expressed gene: | NKG2D |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+1ug/ml puromycin+800ug/ml hygromycin+5ug/ml blasticidin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| III. Representative Data | |
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Figure 1. Recombinant NKG2D Effector Reporter Cell stably expressing NKG2D. |
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Figure 2. Blocking of MICA induced NKG2D Effector Reporter Cells (C5) Activity by NKG2D Blocking Ab with MICA CHO Cells. |
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