hMET/KLN205
CBPG0165
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I. Background | |
C-Met 屬于受體酪氨酸激酶家族,是一種異二聚體,由高度糖基化的細胞外 50 kDa α 鏈和跨膜 140 kDa β鏈組成,通過二硫鍵連接在一起。作為一種天然的 HGF 受體,它表達 于各種上皮細胞的表面,并促進腫瘤的發(fā)生。與 HGF 結合后,兩個亞基二聚化,導致兩個 酪氨酸殘基(Tyr1234 和 Tyr1235 )自磷酸化。酪氨酸殘基的自磷酸化導致一個激活的多功 能??课稽c,該位點由 SH2 結構域、PTB 結構域和 Met 結合結構域 (MDB) 組成,該結 構域募集接頭分子。 | |
II. Introduction | |
Host Cell: | KLN205 |
Expressed gene: | hMET |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: | MEM+10%FBS+1%Non Essential Amino Acids(NEAA)+1mM Sodium Pyruvate(NaP)+10 μg/ml Blasticidin |
Mycoplasma Testing: | Negative |
Storage: | Liquid nitrogen |
III. Description of Host Cell Line | |
Organism: | Mus musculus, mouse |
Tissue: | Lung |
Morphology: | Epithelial |
Growth Properties: | Adherent |
IV. Representative Data | |
Figure 1. Recombinant hMET/KLN205 stably expressing hMET. |
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