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OE33(人食道腺癌腫瘤細(xì)胞)

CBP60496

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I. General information 
Synonyms: OE33
Background: The cell line OE33, also known as JROECL33, was established from the adenocarcinoma of the lower oesophagus (Barrett's metaplasia) of a 73 year old female patient. The tumour was identified as pathological stage IIA (UICC) and showed poor differentiation. OE33 express HLA-A, -B and -C antigens (MHC class I) and ICAM-1 constitutively. Expression of HLA-DR (MHC class II) can be induced by treatment with interferon-gamma. The cells express epithelial cytokeratins and are tumourigenic in nude mice.
Cultures derived from ECACC stocks of this cell line have been whole genome sequenced (Contino et al 2016) confirming the presence of many of the known mutations that drive oesophageal cancer
Species: human (Homo sapiens)
Tissue: oesophagus
Disease: 73 year old female
Gender: Human Caucasian oesophageal carcinoma
Morphology: Epithelial
Growth Mode: Adherent
Doubling Time: N/A
DNA Profile: Amelogenin: X
CSF1PO: 10
D13S317: 14
D16S539: 12
D5S818: 11
D7S820: 9,10
THO1: 7,8
TPOX: 8,11
vWA: 17
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Culture Medium:

RPMI1640+10%FBS

OE33完全培養(yǎng)基,# CBP60496M
We strongly suggest to purchase the complete medium from us.

Cryopreservation medium: 90%FBS+10%DMSO
Karyotype: Aneuploid
Subculture Routine: Split sub-confluent cultures (70-80%) 1:8 i.e. seeding at 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Initially these cells grow slowly and can take up to 7 days until ready for the next split, 50% media changes will be necessary every 2-3 days (i.e. replacing half the old medium with fresh).
Comments: For more information, please contact us (4008-750-250).

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藥靶模型聯(lián)系方式: 華東銷售經(jīng)理(上海):18240630236 華東銷售經(jīng)理(上海、江蘇、安徽):15715191010 華中&華西銷售經(jīng)理:18071545918 華中&西南銷售經(jīng)理:13871580511 華北銷售經(jīng)理:18628311252 全國銷售經(jīng)理:13816461235
診斷標(biāo)準(zhǔn)品聯(lián)系方式: 華東銷售經(jīng)理:15000320447 華北銷售經(jīng)理:18628311252 華中&華西銷售經(jīng)理:18071545918 華中&西南銷售經(jīng)理:13871580511 全國銷售經(jīng)理:13816461235

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