KASUMI-1（人急性粒細(xì)胞白血病細(xì)胞）
CBP60524
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| I. General information | |
| Synonyms: | KASUMI-1 |
| Background: | The cell line was established from the peripheral blood of an acute myeloid leukemia (AML) patient. |
| Species: | Homo sapiens, human |
| Disease: | Acute myeloblastic leukemia |
| Gender: | Male |
| Morphology: | myeloblast |
| Growth Mode: | suspension |
| DNA Profile: | D5S818: 9,11 D13S317: 11,13 D7S820: 8,11 D16S539: 9,12 vWA: 14 THO1: 6,9 Amelogenin: X TPOX: 8,9 CSF1PO: 10,12 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI1640+20%FBS KASUMI-1完全培養(yǎng)基，# CBP60524M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Comments: | This is a leukemic cell line with an 8;21 chromosome translocation. This translocation juxtaposes the AML1 with ETO (or MTG8) gene, giving rise to the fusion gene AML1-ETO (also known as AML1-MTG or RUNX1-CBF2T1), hence the cells produce chimeric AML1-ETO protein. This protein down-regulates CEBPA mRNA, protein and DNA binding activity, which is crucial for the differentiation of granulocytes.The cells are positive for myeloperoxidase showing a morphology of myeloid maturation. In proliferation assay the cells in culture showed response to interleukin-3 (IL-3), IL-6, granulocyte colony-stimulating factor (G-CSF), and granulocytemacrophage CSF (GM-CSF), but not to IL-1 or IL-5. Neither granulocytic nor eosinophilic maturation was observed in the in vitro liquid culture by the addition of dimethyl sulfoxide, G-CSF, or IL-5, respectively. Induction of macrophagelike cells was seen by the addition of phorbol ester. Proliferation is inhibited by 1,25S-(OH)2-16,23-diene-26-F3-10-nor D3. For more information, please contact Cobioer (4008-750-250). |
