M059J（人腦惡性膠質(zhì)母細(xì)胞瘤）
CBP60596
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| I. General information | |
| Synonyms: | M059J |
| Background: | M059J cells were isolated from a tumor specimen taken from a 33 year old male with untreated malignant glioblastoma，The cells were isolated concurrently from the same tumor specimen as M059K (see CRL-2365). |
| Species: | Homo sapiens, human |
| Tissue: | brain |
| Disease: | malignant glioblastoma; glioma |
| Gender: | male, 33 years |
| Morphology: | fibroblast |
| Growth Mode: | adherent |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 14 D16S539: 10,12 D5S818: 11,12 D7S820: 10,12 THO1: 9.3 TPOX: 8 vWA: 17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM/F12 + 10% FBS+1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) M059J完全培養(yǎng)基，# CBP60596M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Comments: | M059J cells lack DNA-dependent protein kinase activity, while M059K cells express normal levels of DNA-dependent protein kinase. M059J cells are approximately 30-fold more sensitive to ionizing radiation than M059K cells. M059J cells are more sensitive than M059K cells to the cytotoxic effects of bleomycin, N,N-bis(2-choroethyl)-N-nitrosourea and nitrogen mustard. M059J cells are deficient in repair of DNA double strand breaks. The cells are negative for glial fibrillary acidic protein (GFAP). For more information, please contact Cobioer (4008-750-250). |
