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NR8383(大鼠肺泡巨噬細胞)

CBP60897

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I. General information 
Synonyms: NR8383
Background: NR8383 (normal rat, August 3, 1983) was established from normal rat alveolar macrophage cells obtained by lung lavage.
NR8383 cells were cloned and subcloned from single cells by limiting dilution, and then subcloned from soft agar three times.
Species: Rattus norvegicus, rat
Tissue: lung
Disease: normal
Morphology: macrophage
Growth Mode: mixed, adherent and suspension
Culture Medium:

F12K+20%FBS

NR8383完全培養(yǎng)基,# CBP60897M
We strongly suggest to purchase the complete medium from us.

Cryopreservation medium: 90%FBS+10%DMSO
Receptor Expression: Fc
Genes Expressed: transforming growth factor beta (TGF beta); interleukin 1 (IL-1); interleukin 6 (IL-6)
Cellular Products: transforming growth factor beta (TGF beta); interleukin 1 (IL-1); interleukin 6 (IL-6)
Comments: The cells were cultured in the presence of gerbil lung cell conditioned medium for approximately 8 to 9 months.
Subsequently the requirement for exogenous growth factors was lost.
The cells exhibit characteristics of macrophage cells.
Phagocytosis of zymosan and Pseudomonas aeruginosa, nonspecific esterase activity, Fc receptors, oxidative burst,IL-1, TNF beta and IL-6 secretion, and replicative response to exogenous growth factors.
The cells respond to appropriate microbial, particulate or soluble stimuli with phagocytosis and killing.
NR8383 cells respond to bleomycin by secreting latent transforming growth factor (TGF beta).
Stimulation with bleomycin also increases TGF beta mRNA expression.
These cells are sensitive to endotoxin.
LPS levels of 1 to 10 ng/mL inhibit replication by 50%.
LPS inhibition is nontoxic and reversible even after levels up to 0.001 mg/mL for extended periods.
For more information, please contact us (4008-750-250).

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