FL 62891（人肝細(xì)胞（SV40轉(zhuǎn)化））
CBP60816
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| I. General information | |
| Synonyms: | FL 62891 |
| Background: | The cell line was derived from human fetal liver tissue, and immortalized by transformation with an ecotropic retrovirus containing a temperature sensitive SV40 T-antigen (tsA58) and a G418 resistance gene. After several days at 37C, the temperature of the medium was lowered to 32C. Cells were selected with G418. The selected cells were expanded and maintained. The cells produce flk-1 and some flk-2 ligands. |
| Species: | Homo sapiens, human |
| Gender: | fetus |
| Morphology: | fibroblast |
| Growth Mode: | adherent |
| Doubling Time: | 48 hours |
| Culture Medium: |
IMDM+10%FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) FL 62891完全培養(yǎng)基，# CBP60816M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Comments: | The cell line was derived from human fetal liver tissue, and immortalized by transformation with an ecotropic retrovirus containing a temperature sensitive SV40 T-antigen (tsA58) and a G418 resistance gene. After several days at 37C, the temperature of the medium was lowered to 32C. Cells were selected with G418. The selected cells were expanded and maintained. The cells produce flk-1 and some flk-2 ligands. For more information, please contact Cobioer (4008-750-250). |
