15P-1（小鼠睪丸上皮細(xì)胞）
CBP60908
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| I. General information | |
| Synonyms: | 15P-1 |
| Background: | The 15P-1 cell line was established from testicular cells of transgenic mice that express the large T protein of polyoma virus (PyLT) in the seminiferous epithelium.Ref |
| Species: | Mus musculus, transgenic, mouse, transgenic |
| Tissue: | testis |
| Gender: | male, 6 months |
| Growth Mode: | adherent |
| Culture Medium: |
DMEM+10%FBS 15P-1完全培養(yǎng)基，# CBP60908M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Comments: | The 15P-1 cell line was established from testicular cells of transgenic mice that express the large T protein of polyoma virus (PyLT) in the seminiferous epithelium. Ref This line exhibits characteristics of Sertoli cells, including transcription of the Wilms' tumor (WT1) and Steel genes. Ref 15P-1 cultures express phagocytic activity evidenced by the internalization of latex beads and the disposal of dead cells. Interaction with germ cells enhances the phagocytic activity of 15P-1 cells. Ref The cells support the meiotic and postmeiotic differentiation in cocultures of diploid premeiotic germ cells into haploid spermatids expressing the protamine (Prm-1) gene. Ref When cocultured with 15P-1 cells, testicular cells explanted from immature 9-day-old animals, before the onset of the first meiosis, generate tetrads of haploid cells with the morphology of round spermatids and initiate protamine transcription. Ref The 15P-1 cell line releases protease-sensitive material with broad-spectrum antibacterial activity. The activity of 15P-1 culture medium is increased 10-fold to 50-fold in the presence of fractions enriched in round spermatids and of nerve growth factor. Ref The KL protein, a ligand of the Kit receptor, is expressed in Sertoli cells and in 15P-1 cells. Ref For more information, please contact Cobioer (4008-750-250). |
