NE-4C （小鼠神經(jīng)干細(xì)胞）
CBP60905
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| I. General information | |
| Synonyms: | NE-4C |
| Background: | The neuroepithelial cell lines, NE-4C (ATCC CRL-2925) and NE-GFP-4C (ATCC CRL-2926) were established from the cerebral vesicles of 9-day-old mouse embryos lacking the functional p53 genes. |
| Species: | Mus musculus, mouse |
| Tissue: | brain, neuroectodermal |
| Gender: | embryo |
| Morphology: | neuroepithelial |
| Growth Mode: | adherent |
| Doubling Time: | about 12 hours |
| Culture Medium: |
MEM+2mM L-Glutamine+10%FBS+1%NEAA+1%NaP NE-4C完全培養(yǎng)基，# CBP60905M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | Sox-2, Otx-2, En-1 |
| Comments: | The cells from both cell lines differentiated to neurons and astrocytes when exposed to retinoic acid. The GFP-transfected clone, NE-GFP-4C, when implanted into the forebrain of adult, new-born, and fetal mice or into the mid- and forebrain vesicles of early chick embryos was capable of developing morphologically differentiated neurons. Ref Ref For more information, please contact Cobioer (4008-750-250). |
