EA.hy926(人臍靜脈細(xì)胞融合A549細(xì)胞)
CBP60714
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I. General information | |
Synonyms: | EA.hy926 |
Background: | The human umbilical vein cell line, EA.hy926, was established by fusing primary human umbilical vein cells with a thioguanine-resistant clone of A549 by exposure to polyethylene glycol (PEG). Hybrid clones were selected in HAT medium and screened for factor VIII-related antigen. |
Species: | Homo sapiens, human |
Tissue: | somatic cell hybrid |
Morphology: | endothelial |
Growth Mode: | adherent |
DNA Profile: | Amelogenin: X CSF1PO: 10,11,12 D13S317: 11 D16S539: 11,12 D5S818: 11 D7S820: 8,9,10 THO1: 6,8,9.3 TPOX: 8,9 vWA: 14,17 Cobioer’s Cell Line Authentication Service |
Culture Medium: |
DMEM+10%FBS EA.hy926完全培養(yǎng)基,# CBP60714M |
Cryopreservation medium: | 90%FBS+10%DMSO |
Antigen Expression: | Factor VIII-related antigen; Homo sapiens, expressed |
Genes Expressed: | Factor VIII-related antigen; Homo sapiens. |
Tumor Formation: | No, in immunosuppressed mice,Yes, in semisolid medium |
Comments: | Electron photomicrographs demonstrate cytoplasmic distribution of Weibel-Palade bodies and tissue-specific organelles, characteristics of differentiated endothelial cell functions such as angiogenesis, homeostasis/thrombosis, blood pressure and inflammation. Ref Ref EA.hy926 cells have been maintained for more than 100 population doublings (PDLs). For more information, please contact Cobioer (4008-750-250). |