NTERA-2 ?（人惡性多發(fā)性畸胎瘤細(xì)胞）
CBP60799
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| I. General information | |
| Synonyms: | NTERA-2 |
| Background: | NTERA-2 was cloned from cell line TERA-2 which was derived from a metastatic teratocarcinoma of a 22-year-old Caucasian male; cell line also known as NT-2 |
| Species: | confirmed as human by cytogenetics |
| Tissue: | Embryo |
| Disease: | embryonal carcinoma (teratocarcinoma) |
| Gender: | 22-year-old Caucasian male |
| Growth Mode: | adherent epitheloid cells carrying strong granula growing adherent in monolayer |
| Doubling Time: | 50-60 hours |
| Culture Medium: |
DMEM+10%FBS NTERA-2完全培養(yǎng)基，# CBP60799M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Immunology: | cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 -, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | Mycoplasma: negative in microbiological culture, PCR assays Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile Cytogenetics: human flat-moded hyperdiploid karyotype - 48-58<2n>XXXY, +1, +16, +20, +22, +3-4mar, add(X)(p11), add(X)(q2?5), der(X;1)(p10;q10), add(1)(p35-36), add(11)(p15), add(13)(q3?1) - sideline with i(3q) For more information, please contact Cobioer (4008-750-250). |
