NCI-H295R（人腎上腺皮質(zhì)腺瘤）
CBP60794
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| I. General information | |
| Synonyms: | NCI-H295R |
| Background: | NCI-H295R was adapted from the NCI-H295 pluripotent adrenocortical carcinoma cell line (ATCC CRL-10296) established by A.F. Gazdar and associates from a carcinoma of the adrenal cortex. The original cells were adapted to a culture medium which decreased the population doubling time from 5 days to 2 days. While the original cells grew in suspension, the adapted cells were selected to grow in a monolayer. This cell line retains the ability to produce adrenal androgens. It is responsive to angiotensin II and potassium ions. |
| Species: | Homo sapiens, human |
| Tissue: | adrenal gland/cortex |
| Disease: | Carcinoma |
| Gender: | female, 48 years, Black |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| DNA Profile: | Amelogenin: X CSF1PO: 10,12 D13S317: 13 D16S539: 11 D5S818: 12 D7S820: 9,12 THO1: 9.3 TPOX: 8 vWA: 17,18 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM:F12 + 0.00625 mg/ml insulin+ 0.00625 mg/ml transferrin;+6.25 ng/ml selenium+1.25 mg/ml bovine serum albumin+ 0.00535 mg/ml linoleic acid; +2.5% Nu-Serum I. The additives NCI-H295R完全培養(yǎng)基，# CBP60794M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Genes Expressed: | aldosterone; cortisol; C19 steroids |
| Cellular Products: | aldosterone; cortisol; C19 steroids |
| Comments: | It is responsive to angiotensin II and potassium ions. This cell line retains the ability to produce adrenal androgens. For more information, please contact Cobioer (4008-750-250). |
