WERI-Rb-1（人視網(wǎng)膜神經(jīng)膠質(zhì)瘤細(xì)胞）
CBP60755
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| I. General information | |
| Synonyms: | WERI-Rb-1 |
| Background: | The WERI-Rb-1 line is one of two human retinoblastoma cell lines established in 1974 by R.M. McFall and T.W. Sery. |
| Species: | Homo sapiens, human |
| Tissue: | eye, retina |
| Disease: | retinoblastoma |
| Gender: | female, 1 year,Caucasian |
| Morphology: | grape-like clusters of round cells |
| Growth Mode: | suspension |
| Culture Medium: |
RPMI-1640+10%FBS WERI-Rb-1完全培養(yǎng)基，# CBP60755M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Tumor Formation: | Yes, in rabbits |
| Comments: | Karyotype This is a near diploid line. The modal chromosome number is 47 occurring at 38%, and the rate of polyploidy is 9%. Fifteen to sixteen marker chromosomes are present in all cells. They are t(1,?), t(3p,5q), der(3)t(?q29;?), t(3q,?), 5q+, i(6p), t(7q,?), 9q+, t(10q,21q), 16q+ and five to six others. Normal chromosomes 3, 10, 13 and 16 are absent. There are two copies of the X chromosome. No Y chromosomes were detected in QM stained preparations The cells survive culturing in Difco Bacto-Agar but do not form colonies. Scanning electron microscopy reveals some variation in the number and frequency of surface blebs, lamellipodia and microvilli. The line is of interest in studies of cell differentiation, animal models of tumor therapy and biochemical evaluations. For more information, please contact Cobioer (4008-750-250). |
