HAL-01（人急性淋巴細(xì)胞白血病細(xì)胞）
CBP61018
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| I. General information | |
| Synonyms: | HAL-01 |
| Background: | established from the peripheral blood of a 17-year-old woman with acute lymphoblastic leukemia (ALL-L2) in 1990; contains the t(17;19)(q22;p13) TCF3-HLF (E2A-HLF) fusion gene |
| Species: | human (Homo sapiens) |
| Tissue: | N/A |
| Disease: | B cell precursor leukemia |
| Gender: | N/A |
| Morphology: | single, round cells in suspension, some cells are slightly adherent; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 48 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS HAL-01完全培養(yǎng)基，# CBP61018M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD38 +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; |
| Fingerprint: | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Species: | confirmed as human by cytogenetics |
| Cytogenetics: | human near-diploid karyotype with 4% polyploidy; 46(43-48)<2n>XX, t(1;7)(p34;q21), t(17;19)(q22;p13); t(17;19) effects rearrangement of TCF3 (E2A) and HLF; matches published karyotype |
| Molec. Genetics: | expression of fusion gene TCF3-HLF (E2A-HLF) confirmed by RT-PCR |
| Virus | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
