CF-1 MEF(小鼠胚胎成纖維細(xì)胞)
CBP61128
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I. General information | |
Synonyms: | CF-1 MEF |
Background: | The cell line was established by ATCC in 2003 from embryonic day 14 (E14) CF-1 mouse embryos. |
Species: | Mus musculus, mouse |
Tissue: | Embryo |
Disease: | N/A |
Gender: | 14 days gestation embryo, male and female mixed |
Morphology: | Fibroblast |
Growth Mode: | adherent |
Doubling Time: | N/A |
DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
Culture Medium: |
DMEM+10%FBS+ 1%Non Essential Amino Acids (NEAA) CF-1 MEF完全培養(yǎng)基,# CBP61128M |
Cryopreservation medium: | 90%FBS+10%DMSO |
Antigen Expression: | N/A |
Receptor Expression: | N/A |
Oncogene: | N/A |
Genes Expressed: | N/A |
Cellular Products: | N/A |
Tumor Formation: | N/A |
Comments: | The growth of these cells should be arrested before being used as a feeder layer. ATCC has successfully irradiated (SCRC-1040.1™) and treated the cells with Mitomycin C (SCRC-1040.2a™) for use as a feeder layer. If the MEFs are being used as a feeder layer for ES cells, it is not recommended to use them past passage no. 6 (P6). For more information, please contact Cobioer (4008-750-250). |