LN229（人腦膠質細胞瘤）
CBP60302
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| I. General information | |
| Synonyms: | LN229 |
| Background: | The LN-229 cell line was established in 1979 from cells taken from a patient with right frontal parieto-occipital glioblastoma [PubMed. 10416987]. |
| Species: | Homo sapiens, human |
| Tissue: | brain/right frontal parieto-occipital cortex |
| Disease: | glioblastoma |
| Gender: | female, 60 years, White |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| DNA Profile: | Amelogenin: X CSF1PO: 12 D13S317: 10,11 D16S539: 12 D5S818: 11,12 D7S820: 8,11 THO1: 9.3 TPOX: 8 vWA: 16,19 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM + 10% FBS LN229完全培養(yǎng)基，# CBP60302M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Oncogene: | p53 + (mutated, CCT (Pro) --> CTT (Leu) mutation at codon 98), PTEN + (wild type), p16 - (deleted), p14ARF - (deleted) |
| Tumor Formation: | Yes, forms tumors in nude mice |
| Comments: | The cells exhibit mutated p53 (TP53) and possible homozygous deletions in the p16 and p14ARF tumor suppressor genes. They have a wild-type PTEN gene. Stimulation of the cells with Fas ligand lead to apoptotic cell death within 16 hours. The cells were also killed by puromycin in a dose dependent manner. Bcl-2 protects these cells from Fas ligand-induced cell death but was shown to have only a small protective effect on puromycin-induced apoptosis. For more information, please contact Cobioer (4008-750-250). |
