OCI-AML-3（人急性髓系白血病細胞）
CBP60817
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| I. General information | |
| Synonyms: | OCI-AML-3 |
| Background: | established from the peripheral blood of a 57-year-old man with acute myeloid leukemia (AML FAB M4) at diagnosis in 1987; cells carry an NPM1 gene mutation (type A) and the DNMT3A R882C mutation |
| Species: | human (Homo sapiens) |
| Tissue: | N/A |
| Disease: | acute myeloid leukemia |
| Gender: | N/A |
| Morphology: | single, mostly round cells growing in suspension; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 35-40 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: | RPMI-1640+20%FBS We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, cyCD68 +, HLA-DR -; |
| Fingerprint: | fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile |
| Species: | confirmed as human by cytogenetics and species PCR |
| Cytogenetics: | human hyperdiploid karyotype - 48(45-50)<2n>X/XY, +1, +5, +8, der(1)t(1;18)(p11;q11), i(5p), del(13)(q13q21), dup(17)(q21q25) - sideline with r(Y)x1-2 - hemizygous for RB1 |
| Virus | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
