COLO-320（人結(jié)腸腺癌細(xì)胞）
CBP61130
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| I. General information | |
| Synonyms: | COLO-320 |
| Background: | established from the tumor mass of a 55-year-old Caucasian woman with a moderately undifferentiated adenocarcinoma of the sigmoid colon in 1977; cells were described in the literature to produce several hormones, e.g. serotonin, epinephrine; cells express MYC mRNA |
| Species: | human (Homo sapiens) |
| Tissue: | colon |
| Disease: | colon adenocarcinoma |
| Gender: | N/A |
| Morphology: | undifferentiated, round cells in suspension and loosely adherent in monolayers; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 38 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS COLO-320完全培養(yǎng)基，# CBP61130M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin + |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of AST, G6PD, MDH, NP |
| Cytogenetics: | human hyperdiploid karyotype - 53(50-54)<2n>XX, -3, -3, +5, +7, +12, -13, -17, +18, +22, +6mar, t(1;5) (p11;q11), der(5)t(5;?)(p15;?), der(7)t(7;?)(q22;?), der(9)t(9;?)(p11;?), del(9)(p22), del(12)(p12), i(13q), der(14)t(14;?)(q24;?), der(18)t(18;?)(p11;?) - variable numbers of supernumerary dmins were present in 90% of cells |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
