CXCR7 β-Arrestin CHO
CBP71468
| I. Background | |
|
趨化因子受體是 G 蛋白偶聯(lián)的七次跨膜細(xì)胞表面受體，其配體與其結(jié)合具有高親合力。 迄今為止，至少有 20 種趨化因子受體已被確認(rèn)，這些受體也可以分為四種亞型：CXC 趨 化因子受體(CXCRs)， CC 趨化因子受體(CCRs)， XCR 和 CX3CR。 |
|
| II. Description | |
|
CXCR7 β-Arrestin CHO 報(bào)告基因藥靶模型很好的模擬了體內(nèi) CXCR7/β-Arrestin 的信號(hào)轉(zhuǎn)導(dǎo)過程，原理見下圖所示。
|
|
| Figure 1. CXCR7 β-Arrestin CHO 細(xì)胞模型原理圖
|
|
| III. Introduction | |
| Expressed gene: | CXCR7 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12k+10%FBS+5μg/ml puromycin+5μg/ml blasticidin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional assay for CXCR7 receptor |
| Transducer: | Gi |
| IV. Representative Data | |
|
Figure 3. Dose response of Recombinant Human CXCL12 in CXCR7 β-Arrestin CHO(C11). |
|
