RKO-E6（結(jié)腸癌細(xì)胞系）
CBP60741
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| I. General information | |
| Synonyms: | RKO-E6 |
| Background: | The RKO-E6 cell line was generated from the colon carcinoma RKO (ATCC CRL-2577) cell line by transfection with pCMV-E6 using Lipofectin. |
| Species: | Homo sapiens, human |
| Tissue: | colon |
| Disease: | Carcinoma, Papilloma |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| Culture Medium: |
MEM+10%FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) RKO-E6完全培養(yǎng)基，# CBP60741M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Comments: | The cells contain a stably integrated human papilloma virus (HPV) E6 oncogene under control of the cytomegalovirus (CMV) promoter. The HPV E6 oncogene causes a decrease in normal p53 levels and functions. The RKO-E6 cell line lacks appreciable functional p53. Disruption of normal p53 function in human colon carcinoma RKO cells with the human papillomavirus E6 oncoprotein results in reduced repair of u.v.-induced DNA damage and also loss of induced repair following cellular u.v.-irradiation. Disruption of normal p53 function in human colon carcinoma RKO cells with the human papillomavirus E6 oncoprotein results in reduced repair of u.v.-induced DNA damage and also loss of induced repair following cellular u.v.-irradiation. The RKO-E6 cell line can be used together with its parental cell line, RKO (ATCC CRL-2577) to investigate the effects of p53 loss on cellular parameters such as p53 mediated transcription and apoptosis. For more information, please contact Cobioer (4008-750-250). |
