EML4-ALK V1 [I1171N/F1174I]/BaF3
CBP73300
| I. Introduction | |
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Cell Line Name： |
EML4-ALK V1 [I1171N/F1174I]/BaF3 |
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Host Cell: |
Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
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Application: |
Anti-proliferation assay and PD assay |
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Freeze Medium: |
90% FBS+10% DMSO |
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Complete Culture Medium: |
RPMI-1640+10%FBS |
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Mycoplasma Status: |
Negative |
| II. Background | |
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EML4-ALK results from the fusion of EML4 and ALK, resulting in constitutive kinase activity, transformation in cultured cells, and tumor activity in mouse models (PMID: 19064915, PMID: 17625570). EML4-ALK fusions have been associated with non-small cell lung cancer (PMID: 26755435). |
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| III. Representative Data | |
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1. WB of EML4-ALK V1 [I1171N/F1174I]/BaF3
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2. Sanger of EML4-ALK V1 [I1171N/F1174I]/BaF3 Figure 2. EML4-ALK V1 [I1171N/F1174I]/BaF3 Fusion
Figure 3. EML4-ALK V1 [I1171N/F1174I]/BaF3 F1174I
Figure 4. EML4-ALK V1 [I1171N/F1174I]/BaF3 I1171N |
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2. Anti-proliferation assay
Figure 5. CTG Proliferation Assay of BaF3 EML4-ALK V1 I1171N/F1174I (C10). |
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